› 1jgt

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF BETA-LACTAM SYNTHETASE

Released:
DOI: 10.2210/pdb1jgt/pdb
PDB entry 1jgt coloured by chain, front view.
PDB entry 1jgt coloured by chain, side view.
PDB entry 1jgt coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
Structure of beta-lactam synthetase reveals how to synthesize antibiotics instead of asparagine.
Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC
Nat Struct Biol 8 684-9 (2001)
PMID: 11473258

Function and Biology Details

Reaction catalysed: 
ATP + L-N(2)-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxyethyl-arginine beta-lactam-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 54.6 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DJQ7 (Residues: 1-513; Coverage: 100%)
Gene name: bls
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand APC 2 x APC
Ligand CMA 2 x CMA
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 61.352Å b: 97.873Å c: 80.969Å
α: 90° β: 90.78° γ: 90°
R-values:
R R work R free
0.228 0.195 0.228
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Asparagine synthetase chemotherapy.
Richards et al. (2006)
10 more

4 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
3 more