› 1jcx

X-ray diffraction
1.8Å resolution

Aquifex aeolicus KDO8P synthase in complex with API and Cadmium

Released:
DOI: 10.2210/pdb1jcx/pdb
PDB entry 1jcx coloured by chain, front view.
PDB entry 1jcx coloured by chain, side view.
PDB entry 1jcx coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structures of Aquifex aeolicus KDO8P synthase in complex with R5P and PEP, and with a bisubstrate inhibitor: role of active site water in catalysis.
Wang J, Duewel HS, Woodard RW, Gatti DL
Biochemistry 40 15676-83 (2001)
PMID: 11747443

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.77 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66496 (Residues: 1-267; Coverage: 100%)
Gene names: aq_085, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CD 2 x CD
Ligand PAI 2 x PAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 84.532Å b: 84.532Å c: 160.199Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.229
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The enzymes of sialic acid biosynthesis.
Tanner ME. (2005)
2 more