› 1jbs

X-ray diffraction
1.97Å resolution

Crystal structure of ribotoxin restrictocin and a 29-mer SRD RNA analog

Released:
DOI: 10.2210/pdb1jbs/pdb
PDB entry 1jbs coloured by chain, front view.
PDB entry 1jbs coloured by chain, side view.
PDB entry 1jbs coloured by chain, top view.
Source organism: Aspergillus restrictus
Primary publication:
Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping.
Yang X, Gérczei T, Glover LT, Correll CC
Nat Struct Biol 8 968-73 (2001)
PMID: 11685244

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159253 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease mitogillin Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.89 KDa
Source organism: Aspergillus restrictus
UniProt:
  • Canonical: P67876 (Residues: 28-176; Coverage: 100%)
Gene name: ret
Structure domains: Microbial ribonucleases
29-mer sarcin/ricin domain RNA analog Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 nucleotides
Theoretical weight: 9.51 KDa
Source organism: Aspergillus restrictus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 6 x K
4 modified residues:
Ligand OMC 10 x OMC
Ligand OMG 10 x OMG
Ligand OMU 2 x OMU
Ligand A2M 2 x A2M

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 62.511Å b: 101.863Å c: 41.092Å
α: 90° β: 92.97° γ: 90°
R-values:
R R work R free
0.217 0.213 0.257
Expression system: Not provided

Quick links

Citations

11 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
10 more

15 mentions without citation

The common and the distinctive features of the bulged-G motif based on a 1.04 A resolution RNA structure.
Correll et al. (2003)
14 more