› 1iz1

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF CBNR, A LYSR FAMILY TRANSCRIPTIONAL REGULATOR

Released:
DOI: 10.2210/pdb1iz1/pdb
PDB entry 1iz1 coloured by chain, front view.
PDB entry 1iz1 coloured by chain, side view.
PDB entry 1iz1 coloured by chain, top view.
Source organism: Cupriavidus necator
Primary publication:
Crystal structure of a full-length LysR-type transcriptional regulator, CbnR: unusual combination of two subunit forms and molecular bases for causing and changing DNA bend.
Muraoka S, Okumura R, Ogawa N, Nonaka T, Miyashita K, Senda T
J Mol Biol 328 555-66 (2003)
PMID: 12706716

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lysR-type domain-containing protein Chains: A, B, P, Q
Molecule details ›
Chains: A, B, P, Q
Length: 294 amino acids
Theoretical weight: 32.09 KDa
Source organism: Cupriavidus necator
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WXC7 (Residues: 1-294; Coverage: 100%)
Gene names: BJN34_37375, cbnR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P212121
Unit cell:
a: 65.271Å b: 124.477Å c: 166.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins.
Maddocks et al. (2008)
7 more

11 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
10 more