› 1iwg

X-ray diffraction
3.5Å resolution

Crystal structure of Bacterial Multidrug Efflux transporter AcrB

Released:
DOI: 10.2210/pdb1iwg/pdb
PDB entry 1iwg coloured by chain, front view.
PDB entry 1iwg coloured by chain, side view.
PDB entry 1iwg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of bacterial multidrug efflux transporter AcrB.
Murakami S, Nakashima R, Yamashita E, Yamaguchi A
Nature 419 587-93 (2002)
PMID: 12374972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1053 amino acids
Theoretical weight: 114.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: R32
Unit cell:
a: 144.536Å b: 144.536Å c: 519.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.29 0.29 0.355
Expression system: Escherichia coli K-12

Quick links

Citations

151 review citations

Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H. (2003)
150 more

47 mentions without citation

Mechanisms of RND multidrug efflux pumps.
Nikaido et al. (2009)
46 more