› 1iw0

X-ray diffraction
1.4Å resolution

Crystal structure of a heme oxygenase (HmuO) from Corynebacterium diphtheriae complexed with heme in the ferric state

Released:
DOI: 10.2210/pdb1iw0/pdb
PDB entry 1iw0 coloured by chain, front view.
PDB entry 1iw0 coloured by chain, side view.
PDB entry 1iw0 coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
The crystal structures of the ferric and ferrous forms of the heme complex of HmuO, a heme oxygenase of Corynebacterium diphtheriae.
Hirotsu S, Chu GC, Unno M, Lee DS, Yoshida T, Park SY, Shiro Y, Ikeda-Saito M
J Biol Chem 279 11937-47 (2004)
PMID: 14645223

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heme oxygenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 215 amino acids
Theoretical weight: 24.17 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71119 (Residues: 1-215; Coverage: 100%)
Gene names: DIP1669, hmuO
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments


Cofactor: Ligand HEM 3 x HEM
Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P21
Unit cell:
a: 53.559Å b: 62.838Å c: 107.661Å
α: 90° β: 100.86° γ: 90°
R-values:
R R work R free
0.168 0.165 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Bacterial iron sources: from siderophores to hemophores.
Wandersman et al. (2004)
16 more

13 mentions without citation

Light-driven catalysis with engineered enzymes and biomimetic systems.
Edwards et al. (2020)
12 more