› 1isz

X-ray diffraction
2Å resolution

Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with galactose

Released:
DOI: 10.2210/pdb1isz/pdb
PDB entry 1isz coloured by chain, front view.
PDB entry 1isz coloured by chain, side view.
PDB entry 1isz coloured by chain, top view.
Source organism: Streptomyces olivaceoviridis
Primary publication:
Crystal structures of the sugar complexes of Streptomyces olivaceoviridis E-86 xylanase: sugar binding structure of the family 13 carbohydrate binding module.
Fujimoto Z, Kuno A, Kaneko S, Kobayashi H, Kusakabe I, Mizuno H
J Mol Biol 316 65-78 (2002)
PMID: 11829503

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.79 KDa
Source organism: Streptomyces olivaceoviridis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SI98 (Residues: 1-436; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GAL 4 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 79.62Å b: 95.14Å c: 141.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.207
Expression system: Escherichia coli

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Citations

5 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
4 more

4 mentions without citation

Energetics of galactose- and glucose-aromatic amino acid interactions: implications for binding in galactose-specific proteins.
Sujatha et al. (2004)
3 more