› 1ise

X-ray diffraction
2.2Å resolution

Crystal structure of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly

Released:
DOI: 10.2210/pdb1ise/pdb
PDB entry 1ise coloured by chain, front view.
PDB entry 1ise coloured by chain, side view.
PDB entry 1ise coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Nakano H, Yoshida T, Oka S, Uchiyama S, Nishina K, Ohkubo T, Kato H, Yamagata Y, Kobayashi Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-recycling factor Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A805 (Residues: 1-185; Coverage: 100%)
Gene names: JW0167, b0172, frr, rrf
Sequence domains: Ribosome recycling factor
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 46.024Å b: 49.267Å c: 49.365Å
α: 90° β: 110.1° γ: 90°
R-values:
R R work R free
0.239 0.239 0.297
Expression system: Escherichia coli BL21(DE3)

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