› 1i9z

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION

Released:
DOI: 10.2210/pdb1i9z/pdb
PDB entry 1i9z coloured by chain, front view.
PDB entry 1i9z coloured by chain, side view.
PDB entry 1i9z coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase.
Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH
Cell 105 379-89 (2001)
PMID: 11348594

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-1,4,5-trisphosphate 5-phosphatase 1 Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 40.09 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43001 (Residues: 534-880; Coverage: 32%)
Gene names: SPBC2G2.02, syj1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 2IP 1 x 2IP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 148.999Å b: 67.705Å c: 51.995Å
α: 90° β: 106.152° γ: 90°
R-values:
R R work R free
0.191 0.191 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
21 more

13 mentions without citation

The Cytolethal Distending Toxin Contributes to Microbial Virulence and Disease Pathogenesis by Acting As a Tri-Perditious Toxin.
Scuron et al. (2016)
12 more