› 1i9g

X-ray diffraction
1.98Å resolution

CRYSTAL STRUCTURE OF AN ADOMET DEPENDENT METHYLTRANSFERASE

Released:
DOI: 10.2210/pdb1i9g/pdb
PDB entry 1i9g coloured by chain, front view.
PDB entry 1i9g coloured by chain, side view.
PDB entry 1i9g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Rv2118c: an AdoMet-dependent methyltransferase from Mycobacterium tuberculosis H37Rv.
Gupta A, Kumar PH, Dineshkumar TK, Varshney U, Subramanya HS
J Mol Biol 312 381-91 (2001)
PMID: 11554794

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-161444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (adenine(58)-N(1))-methyltransferase TrmI Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 30.16 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFZ1 (Residues: 1-280; Coverage: 100%)
Gene names: Rv2118c, trmI
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 66.42Å b: 86.79Å c: 138.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.
Guy et al. (2014)
5 more

11 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
10 more