› 1i84

Electron crystallography
20Å resolution

CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.

Released:
DOI: 10.2210/pdb1i84/pdb
PDB entry 1i84 coloured by chain, front view.
PDB entry 1i84 coloured by chain, side view.
PDB entry 1i84 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2.
Wendt T, Taylor D, Trybus KM, Taylor K
Proc Natl Acad Sci U S A 98 4361-6 (2001)
PMID: 11287639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135958 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin-11 Chains: S, V
Molecule details ›
Chains: S, V
Length: 1184 amino acids
Theoretical weight: 137.07 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10587 (Residues: 2-1175; Coverage: 59%)
Gene name: MYH11
Sequence domains:
Myosin light polypeptide 6 Chains: T, W
Molecule details ›
Chains: T, W
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 2-151; Coverage: 99%)
Gene name: MYL6
Myosin regulatory light chain 11 Chains: U, Z
Molecule details ›
Chains: U, Z
Length: 166 amino acids
Theoretical weight: 18.33 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02609 (Residues: 3-168; Coverage: 99%)
Gene names: MYL11, MYLPF, RLC

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 8 x MLY

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Expression system: Spodoptera frugiperda

Quick links

Citations

41 review citations

Non-muscle myosin II takes centre stage in cell adhesion and migration.
Vicente-Manzanares et al. (2009)
40 more

28 mentions without citation

Lessons from a tarantula: new insights into muscle thick filament and myosin interacting-heads motif structure and function.
Alamo et al. (2017)
27 more