› 1i6l

X-ray diffraction
1.72Å resolution

1.7 HIGH RESOLUTION EXPERIMENTAL PHASES FOR TRYPTOPHANYL-TRNA SYNTHETASE COMPLEXED WITH TRYPTOPHANYL-5'AMP

Released:
DOI: 10.2210/pdb1i6l/pdb
PDB entry 1i6l coloured by chain, front view.
PDB entry 1i6l coloured by chain, side view.
PDB entry 1i6l coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
High-resolution experimental phases for tryptophanyl-tRNA synthetase (TrpRS) complexed with tryptophanyl-5'AMP.
Retailleau P, Yin Y, Hu M, Roach J, Bricogne G, Vonrhein C, Roversi P, Blanc E, Sweet RM, Carter CW
Acta Crystallogr D Biol Crystallogr 57 1595-608 (2001)
PMID: 11679724

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.69 KDa
Source organism: Geobacillus stearothermophilus
UniProt:
  • Canonical: P00953 (Residues: 1-328; Coverage: 100%)
Gene name: trpS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 7 x SO4
Ligand NH4 1 x NH4
Ligand TYM 1 x TYM
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 59.77Å b: 59.77Å c: 232.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.241

Quick links

Citations

2 review citations

Emergence and evolution.
Bullwinkle et al. (2014)
1 more

12 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
11 more