› 1i2b

X-ray diffraction
1.75Å resolution

CRYSTAL STRUCTURE OF MUTANT T145A SQD1 PROTEIN COMPLEX WITH NAD AND UDP-SULFOQUINOVOSE/UDP-GLUCOSE

Released:
DOI: 10.2210/pdb1i2b/pdb
PDB entry 1i2b coloured by chain, front view.
PDB entry 1i2b coloured by chain, side view.
PDB entry 1i2b coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Theisen MJ, Sanda SL, Ginell SL, Benning C, Garavito RM

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-sulfoquinovose + H(2)O = UDP-alpha-D-glucose + sulfite
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-129255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-sulfoquinovose synthase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 45.48 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O48917 (Residues: 86-477; Coverage: 82%)
Gene names: At4g33030, F26P21.150, SQD1
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
3 bound ligands:
Ligand SO4 2 x SO4
Ligand UPG 1 x UPG
Ligand USQ 1 x USQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4122
Unit cell:
a: 159.644Å b: 159.644Å c: 99.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.196
Expression system: Escherichia coli

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