› 1hop

X-ray diffraction
2.3Å resolution

STRUCTURE OF GUANINE NUCLEOTIDE (GPPCP) COMPLEX OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI AT PH 6.5 AND 25 DEGREES CELSIUS

Released:
DOI: 10.2210/pdb1hop/pdb
PDB entry 1hop coloured by chain, front view.
PDB entry 1hop coloured by chain, side view.
PDB entry 1hop coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Refined crystal structures of guanine nucleotide complexes of adenylosuccinate synthetase from Escherichia coli.
Poland BW, Hou Z, Bruns C, Fromm HJ, Honzatko RB
J Biol Chem 271 15407-13 (1996)
PMID: 8663109

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 47.27 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0A7D4 (Residues: 2-432; Coverage: 100%)
Gene names: JW4135, adeK, b4177, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GCP 2 x GCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 73.85Å b: 94.06Å c: 119.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.227
Expression system: Not provided

Quick links

Citations

1 review citation

Structure-function studies of adenylosuccinate synthetase from Escherichia coli.
Honzatko et al. (1999)
8 other articles

2 mentions without citation

sc-PDB: a 3D-database of ligandable binding sites--10 years on.
Desaphy et al. (2015)
1 more