› 1hn4

X-ray diffraction
1.5Å resolution

PROPHOSPHOLIPASE A2 DIMER COMPLEXED WITH MJ33, SULFATE, AND CALCIUM

Released:
DOI: 10.2210/pdb1hn4/pdb
PDB entry 1hn4 coloured by chain, front view.
PDB entry 1hn4 coloured by chain, side view.
PDB entry 1hn4 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The basis for k(cat) impairment in prophospholipase A(2) from the anion-assisted dimer structure.
Epstein TM, Yu BZ, Pan YH, Tutton SP, Maliwal BP, Jain MK, Bahnson BJ
Biochemistry 40 11411-22 (2001)
PMID: 11560489

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, major isoenzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.77 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00592 (Residues: 16-146; Coverage: 100%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 5 x SO4
Ligand MJI 1 x MJI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 37.488Å b: 54.852Å c: 56.896Å
α: 90° β: 104.058° γ: 90°
R-values:
R R work R free
0.217 0.215 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure, function and interfacial allosterism in phospholipase A2: insight from the anion-assisted dimer.
Bahnson BJ. (2005)
6 other articles

5 mentions without citation

Harvesting candidate genes responsible for serious adverse drug reactions from a chemical-protein interactome.
Yang et al. (2009)
4 more