› 1hg7

X-ray diffraction
1.15Å resolution

High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus

Released:
DOI: 10.2210/pdb1hg7/pdb
PDB entry 1hg7 coloured by chain, front view.
PDB entry 1hg7 coloured by chain, side view.
PDB entry 1hg7 coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
Understanding the mechanism of ice binding by type III antifreeze proteins.
Antson AA, Smith DJ, Roper DI, Lewis S, Caves LS, Verma CS, Buckley SL, Lillford PJ, Hubbard RE
J Mol Biol 305 875-89 (2001)
PMID: 11162099

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-3 ice-structuring protein HPLC 12 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.04 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19614 (Residues: 1-65; Coverage: 99%)
Structure domains: Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 32.218Å b: 39.127Å c: 44.694Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 not available 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Antifreeze proteins: an unusual receptor-ligand interaction.
Jia et al. (2002)
7 more

20 mentions without citation

From ice-binding proteins to bio-inspired antifreeze materials.
Voets IK. (2017)
19 more