› 1hc8

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEX

Released:
DOI: 10.2210/pdb1hc8/pdb
PDB entry 1hc8 coloured by chain, front view.
PDB entry 1hc8 coloured by chain, side view.
PDB entry 1hc8 coloured by chain, top view.
Source organisms:
Primary publication:
A compact RNA tertiary structure contains a buried backbone-K+ complex.
Conn GL, Gittis AG, Lattman EE, Misra VK, Draper DE
J Mol Biol 318 963-73 (2002)
PMID: 12054794

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157427 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56210 (Residues: 58-133; Coverage: 57%)
Gene name: rplK
Sequence domains: Ribosomal protein L11, RNA binding domain
Structure domains: Ribosomal protein L11, C-terminal domain
58 NUCLEOTIDE RIBOSOMAL 23S RNA DOMAIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 58 nucleotides
Theoretical weight: 18.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

3 bound ligands:
Ligand MG 21 x MG
Ligand K 2 x K
Ligand OS 4 x OS
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 150.675Å b: 150.675Å c: 63.841Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

16 review citations

A guide to ions and RNA structure.
Draper DE. (2004)
15 more

34 mentions without citation

RNA folding: thermodynamic and molecular descriptions of the roles of ions.
Draper DE. (2008)
33 more