› 1gar

X-ray diffraction
1.96Å resolution

TOWARDS STRUCTURE-BASED DRUG DESIGN: CRYSTAL STRUCTURE OF A MULTISUBSTRATE ADDUCT COMPLEX OF GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE AT 1.96 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1gar/pdb
PDB entry 1gar coloured by chain, front view.
PDB entry 1gar coloured by chain, side view.
PDB entry 1gar coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution.
Klein C, Chen P, Arevalo JH, Stura EA, Marolewski A, Warren MS, Benkovic SJ, Wilson IA
J Mol Biol 249 153-75 (1995)
PMID: 7776369

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P08179 (Residues: 1-212; Coverage: 100%)
Gene names: JW2485, b2500, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand U89 2 x U89
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 75.7Å b: 73.3Å c: 35.5Å
α: 90° β: 94.3° γ: 90°
R-values:
R R work R free
0.168 0.168 0.29
Expression system: Not provided

Quick links

Citations

5 review citations

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
4 more

3 mentions without citation

Identifying residue-residue clashes in protein hybrids by using a second-order mean-field approach.
Moore et al. (2003)
2 more