› 1g9g

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase F Chain: A
Molecule details ›
Chain: A
Length: 629 amino acids
Theoretical weight: 70.87 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37698 (Residues: 30-658; Coverage: 91%)
Gene names: Ccel_0729, celCCF
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 61.53Å b: 84.77Å c: 122.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
1 more

5 mentions without citation

Molecular and biochemical analyses of CbCel9A/Cel48A, a highly secreted multi-modular cellulase by Caldicellulosiruptor bescii during growth on crystalline cellulose.
Yi et al. (2013)
4 more