› 1g8h

X-ray diffraction
2.8Å resolution

ATP SULFURYLASE FROM S. CEREVISIAE: THE TERNARY PRODUCT COMPLEX WITH APS AND PPI

Released:
DOI: 10.2210/pdb1g8h/pdb
PDB entry 1g8h coloured by chain, front view.
PDB entry 1g8h coloured by chain, side view.
PDB entry 1g8h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation.
Ullrich TC, Blaesse M, Huber R
EMBO J 20 316-29 (2001)
PMID: 11157739

Function and Biology Details

Reaction catalysed: 
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfate adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 511 amino acids
Theoretical weight: 57.82 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08536 (Residues: 1-511; Coverage: 100%)
Gene names: J1436, MET3, YJR010W
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand CD 11 x CD
Ligand CA 6 x CA
Ligand NA 12 x NA
Ligand MG 2 x MG
Ligand ADX 2 x ADX
Ligand POP 2 x POP
Ligand ACY 15 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R32
Unit cell:
a: 186.011Å b: 186.011Å c: 223.812Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.245

Quick links

Citations

10 review citations

Sulfur assimilation and glutathione metabolism under cadmium stress in yeast, protists and plants.
Mendoza-Cózatl et al. (2005)
9 more

3 mentions without citation

Combining specificity determining and conserved residues improves functional site prediction.
Kalinina et al. (2009)
2 more