› 1g74

X-ray diffraction
1.7Å resolution

Toward changing specificity: adipocyte lipid binding protein mutant, oleic acid bound form

Released:
DOI: 10.2210/pdb1g74/pdb
PDB entry 1g74 coloured by chain, front view.
PDB entry 1g74 coloured by chain, side view.
PDB entry 1g74 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Specificity determinants for lipids bound to beta-barrel proteins.
Reese AJ, Banaszak LJ
J Lipid Res 45 232-43 (2004)
PMID: 14594993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid-binding protein, adipocyte Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04117 (Residues: 2-132; Coverage: 99%)
Gene names: Ap2, Fabp4
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand OLA 1 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 118.92Å b: 36.89Å c: 27.87Å
α: 90° β: 92.61° γ: 90°
R-values:
R R work R free
0.208 0.208 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Fatty Acid Signaling Mechanisms in Neural Cells: Fatty Acid Receptors.
Falomir-Lockhart et al. (2019)
1 more

3 mentions without citation

Dewetting transitions in protein cavities.
Young et al. (2010)
2 more