› 1g71

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Released:
DOI: 10.2210/pdb1g71/pdb
PDB entry 1g71 coloured by chain, front view.
PDB entry 1g71 coloured by chain, side view.
PDB entry 1g71 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of a DNA-dependent RNA polymerase (DNA primase).
Augustin MA, Huber R, Kaiser JT
Nat Struct Biol 8 57-61 (2001)
PMID: 11135672

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase small subunit PriS Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 40.84 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P9H1 (Residues: 1-347; Coverage: 100%)
Gene names: PF0110, priA, priS
Sequence domains: DNA primase small subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 6 x CL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 129.883Å b: 136.326Å c: 61.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

DNA replication in the archaea.
Barry et al. (2006)
9 more

7 mentions without citation

Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?
Boudet et al. (2015)
6 more