› 1g5f

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.17 KDa
Source organism: Sphingomonas paucimobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 1-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand CL 1 x CL
Ligand DCE 1 x DCE
1 modified residue:
Ligand 2MR 1 x 2MR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 50.86Å b: 71.89Å c: 73.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.198
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.
Lal et al. (2010)
4 more

1 mention without citation

CAVER: a new tool to explore routes from protein clefts, pockets and cavities.
Petrek et al. (2006)