› 1g01

X-ray diffraction
1.9Å resolution

ALKALINE CELLULASE K CATALYTIC DOMAIN

Released:
DOI: 10.2210/pdb1g01/pdb
PDB entry 1g01 coloured by chain, front view.
PDB entry 1g01 coloured by chain, side view.
PDB entry 1g01 coloured by chain, top view.
Source organism: Bacillus sp. KSM-635
Primary publication:
Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme.
Shirai T, Ishida H, Noda J, Yamane T, Ozaki K, Hakamada Y, Ito S
J Mol Biol 310 1079-87 (2001)
PMID: 11501997

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 40.37 KDa
Source organism: Bacillus sp. KSM-635
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P19424 (Residues: 228-584; Coverage: 39%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand CD 10 x CD
Ligand ACY 5 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3221
Unit cell:
a: 98.2Å b: 98.2Å c: 122Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.227
Expression system: Bacillus subtilis

Quick links

Citations

3 review citations

Industrial applications of alkaliphiles and their enzymes--past, present and future.
Fujinami et al. (2010)
2 more

7 mentions without citation

The Human Antibody Response to the Influenza Virus Neuraminidase Following Infection or Vaccination.
Rajendran et al. (2021)
6 more