› 1fae

X-ray diffraction
2Å resolution

Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellobiose

Released:
DOI: 10.2210/pdb1fae/pdb
PDB entry 1fae coloured by chain, front view.
PDB entry 1fae coloured by chain, side view.
PDB entry 1fae coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action.
Parsiegla G, Reverbel-Leroy C, Tardif C, Belaich JP, Driguez H, Haser R
Biochemistry 39 11238-46 (2000)
PMID: 10985769

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase F Chain: A
Molecule details ›
Chain: A
Length: 629 amino acids
Theoretical weight: 70.87 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37698 (Residues: 30-658; Coverage: 91%)
Gene names: Ccel_0729, celCCF
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 61.44Å b: 84.69Å c: 121.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.151 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Microbial cellulose utilization: fundamentals and biotechnology.
Lynd et al. (2002)
8 more

4 mentions without citation

Conformational diversity analysis reveals three functional mechanisms in proteins.
Monzon et al. (2017)
3 more