› 1f6b

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX

Released:
DOI: 10.2210/pdb1f6b/pdb
PDB entry 1f6b coloured by chain, front view.
PDB entry 1f6b coloured by chain, side view.
PDB entry 1f6b coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Crystal structure of Sar1-GDP at 1.7 A resolution and the role of the NH2 terminus in ER export.
Huang M, Weissman JT, Beraud-Dufour S, Luan P, Wang C, Chen W, Aridor M, Wilson IA, Balch WE
J Cell Biol 155 937-48 (2001)
PMID: 11739406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193031 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small COPII coat GTPase SAR1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.44 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QVY3 (Residues: 1-198; Coverage: 100%)
Gene names: SAR1, SAR1B, SARA2
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand SO4 5 x SO4
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 53.368Å b: 61.689Å c: 71.142Å
α: 90° β: 107.49° γ: 90°
R-values:
R R work R free
0.22 0.22 0.24
Expression system: Escherichia coli

Quick links

Citations

42 review citations

The mechanisms of vesicle budding and fusion.
Bonifacino et al. (2004)
41 more

12 mentions without citation

Probing intracellular vesicle trafficking and membrane remodelling by cryo-EM.
Mehrani et al. (2022)
11 more