› 1ez0

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE NADP+ DEPENDENT ALDEHYDE DEHYDROGENASE FROM VIBRIO HARVEYI.

Released:
DOI: 10.2210/pdb1ez0/pdb
PDB entry 1ez0 coloured by chain, front view.
PDB entry 1ez0 coloured by chain, side view.
PDB entry 1ez0 coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Crystal structure of the NADP+-dependent aldehyde dehydrogenase from Vibrio harveyi: structural implications for cofactor specificity and affinity.
Ahvazi B, Coulombe R, Delarge M, Vedadi M, Zhang L, Meighen E, Vrielink A
Biochem J 349 Pt 3 853-61 (2000)
PMID: 10903148

Function and Biology Details

Reaction catalysed: 
An aldehyde + NADP(+) + H(2)O = a carboxylate + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent fatty aldehyde dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 510 amino acids
Theoretical weight: 54.51 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56694 (Residues: 1-510; Coverage: 100%)
Gene name: aldH
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 79.38Å b: 131.18Å c: 92.54Å
α: 90° β: 92.49° γ: 90°
R-values:
R R work R free
0.215 0.215 0.246
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Toward biotechnological production of adipic acid and precursors from biorenewables.
Polen et al. (2013)
2 more

4 mentions without citation

Mirrors in the PDB: left-handed alpha-turns guide design with D-amino acids.
Annavarapu et al. (2009)
3 more