› 1ex7

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF YEAST GUANYLATE KINASE IN COMPLEX WITH GUANOSINE-5'-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1ex7/pdb
PDB entry 1ex7 coloured by chain, front view.
PDB entry 1ex7 coloured by chain, side view.
PDB entry 1ex7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of unligated guanylate kinase from yeast reveals GMP-induced conformational changes.
Blaszczyk J, Li Y, Yan H, Ji X
J Mol Biol 307 247-57 (2001)
PMID: 11243817

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15454 (Residues: 2-187; Coverage: 100%)
Gene names: D9461.39, GUK1, YDR454C
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand 5GP 1 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P43212
Unit cell:
a: 50.625Å b: 50.625Å c: 155.327Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The ß subunit of voltage-gated Ca2+ channels.
Buraei et al. (2010)
2 more

18 mentions without citation

Guanylate kinase domains of the MAGUK family scaffold proteins as specific phospho-protein-binding modules.
Zhu et al. (2011)
17 more