› 1ehy

X-ray diffraction
2.1Å resolution

X-ray structure of the epoxide hydrolase from agrobacterium radiobacter ad1

Released:
DOI: 10.2210/pdb1ehy/pdb
PDB entry 1ehy coloured by chain, front view.
PDB entry 1ehy coloured by chain, side view.
PDB entry 1ehy coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
The x-ray structure of epoxide hydrolase from Agrobacterium radiobacter AD1. An enzyme to detoxify harmful epoxides.
Nardini M, Ridder IS, Rozeboom HJ, Kalk KH, Rink R, Janssen DB, Dijkstra BW
J Biol Chem 274 14579-86 (1999)
PMID: 10329649

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 294 amino acids
Theoretical weight: 34.07 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31243 (Residues: 1-294; Coverage: 100%)
Gene name: echA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 146.624Å b: 100.201Å c: 96.879Å
α: 90° β: 100.68° γ: 90°
R-values:
R R work R free
0.19 0.19 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Alpha/beta hydrolase fold enzymes: the family keeps growing.
Nardini et al. (1999)
16 more

19 mentions without citation

Principles and characteristics of biological assemblies in experimentally determined protein structures.
Xu et al. (2019)
18 more