› 1e3u

X-ray diffraction
1.66Å resolution

MAD structure of OXA10 class D beta-lactamase

Released:
DOI: 10.2210/pdb1e3u/pdb
PDB entry 1e3u coloured by chain, front view.
PDB entry 1e3u coloured by chain, side view.
PDB entry 1e3u coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa.
Maveyraud L, Golemi D, Kotra LP, Tranier S, Vakulenko S, Mobashery S, Samama JP
Structure 8 1289-98 (2000)
PMID: 11188693

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146959 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase OXA-10 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 246 amino acids
Theoretical weight: 27.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 21-266; Coverage: 100%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily
Beta-lactamase OXA-10 Chain: D
Molecule details ›
Chain: D
Length: 246 amino acids
Theoretical weight: 27.51 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 21-266; Coverage: 100%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand AUC 8 x AUC
Ligand SO4 15 x SO4
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21
Unit cell:
a: 65.53Å b: 82.94Å c: 101.42Å
α: 90° β: 95.03° γ: 90°
R-values:
R R work R free
0.181 0.181 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
14 more

5 mentions without citation

EstB from Burkholderia gladioli: a novel esterase with a beta-lactamase fold reveals steric factors to discriminate between esterolytic and beta-lactam cleaving activity.
Wagner et al. (2002)
4 more