› 1e39

X-ray diffraction
1.8Å resolution

Flavocytochrome C3 from Shewanella frigidimarina histidine 365 mutated to alanine

Released:
DOI: 10.2210/pdb1e39/pdb
PDB entry 1e39 coloured by chain, front view.
PDB entry 1e39 coloured by chain, side view.
PDB entry 1e39 coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
Identification of the active site acid/base catalyst in a bacterial fumarate reductase: a kinetic and crystallographic study.
Doherty MK, Pealing SL, Miles CS, Moysey R, Taylor P, Walkinshaw MD, Reid GA, Chapman SK
Biochemistry 39 10695-701 (2000)
PMID: 10978153

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate reductase (cytochrome) Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 60.56 KDa
Source organism: Shewanella frigidimarina
Expression system: Shewanella frigidimarina
UniProt:
  • Canonical: P0C278 (Residues: 1-571; Coverage: 100%)
Gene names: fcc3, fccA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEC 4 x HEC
3 bound ligands:
Ligand FUM 1 x FUM
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 45.571Å b: 92.172Å c: 78.489Å
α: 90° β: 91.08° γ: 90°
R-values:
R R work R free
0.181 not available 0.247
Expression system: Shewanella frigidimarina

Quick links

Citations

8 review citations

Function and structure of complex II of the respiratory chain.
Cecchini G. (2003)
7 more

4 mentions without citation

Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel et al. (2018)
3 more