› 1e2o

X-ray diffraction
3Å resolution

CATALYTIC DOMAIN FROM DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE

Released:
DOI: 10.2210/pdb1e2o/pdb
PDB entry 1e2o coloured by chain, front view.
PDB entry 1e2o coloured by chain, side view.
PDB entry 1e2o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the truncated cubic core component of the Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex.
Knapp JE, Mitchell DT, Yazdi MA, Ernst SR, Reed LJ, Hackert ML
J Mol Biol 280 655-68 (1998)
PMID: 9677295

Function and Biology Details

Reaction catalysed: 
Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFG6 (Residues: 173-405; Coverage: 58%)
Gene names: JW0716, b0727, sucB
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: F432
Unit cell:
a: 222.8Å b: 222.8Å c: 222.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.249
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
Perham RN. (2000)
5 more

7 mentions without citation

A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex.
Kato et al. (2006)
6 more