› 1dwv

X-ray diffraction
2.35Å resolution

MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-hydroxyarginine and 4-amino tetrahydrobiopterin

Released:
DOI: 10.2210/pdb1dwv/pdb
PDB entry 1dwv coloured by chain, front view.
PDB entry 1dwv coloured by chain, side view.
PDB entry 1dwv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of the N(omega)-hydroxy-L-arginine complex of inducible nitric oxide synthase oxygenase dimer with active and inactive pterins.
Crane BR, Arvai AS, Ghosh S, Getzoff ED, Stuehr DJ, Tainer JA
Biochemistry 39 4608-21 (2000)
PMID: 10769116

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 48.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29477 (Residues: 77-496; Coverage: 37%)
Gene names: Inosl, Nos2
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
4 bound ligands:
Ligand 4AB 2 x 4AB
Ligand ZN 2 x ZN
Ligand SO4 8 x SO4
Ligand HAR 2 x HAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6122
Unit cell:
a: 212.98Å b: 212.98Å c: 114.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.286
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Nitric oxide synthases: structure, function and inhibition.
Alderton et al. (2001)
12 more

5 mentions without citation

Use of Computational Biochemistry for Elucidating Molecular Mechanisms of Nitric Oxide Synthase.
Bignon et al. (2019)
4 more