› 1do2

X-ray diffraction
4Å resolution

TRIGONAL CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1do2/pdb
PDB entry 1do2 coloured by chain, front view.
PDB entry 1do2 coloured by chain, side view.
PDB entry 1do2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structures of HsIU and the ATP-dependent protease HsIU-HsIV.
Bochtler M, Hartmann C, Song HK, Bourenkov GP, Bartunik HD, Huber R
Nature 403 800-5 (2000)
PMID: 10693812

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent protease ATPase subunit HslU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 442 amino acids
Theoretical weight: 49.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6H5 (Residues: 2-443; Coverage: 100%)
Gene names: JW3902, b3931, hslU, htpI
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P321
Unit cell:
a: 201.777Å b: 201.777Å c: 171.628Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.285
Expression system: Escherichia coli BL21(DE3)

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Citations

68 review citations

Recognition and processing of ubiquitin-protein conjugates by the proteasome.
Finley D. (2009)
67 more

11 mentions without citation

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
10 more