› 1cqj

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF DEPHOSPHORYLATED E. COLI SUCCINYL-COA SYNTHETASE

Released:
DOI: 10.2210/pdb1cqj/pdb
PDB entry 1cqj coloured by chain, front view.
PDB entry 1cqj coloured by chain, side view.
PDB entry 1cqj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
ADP-binding site of Escherichia coli succinyl-CoA synthetase revealed by x-ray crystallography.
Joyce MA, Fraser ME, James MN, Bridger WA, Wolodko WT
Biochemistry 39 17-25 (2000)
PMID: 10625475

Function and Biology Details

Reaction catalysed: 
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141656 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP-forming] subunit alpha Chains: A, D
Molecule details ›
Chains: A, D
Length: 286 amino acids
Theoretical weight: 29.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGE9 (Residues: 2-287; Coverage: 99%)
Gene names: JW0718, b0729, sucD
Sequence domains:
Structure domains:
Succinate--CoA ligase [ADP-forming] subunit beta Chains: B, E
Molecule details ›
Chains: B, E
Length: 385 amino acids
Theoretical weight: 41.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A836 (Residues: 1-385; Coverage: 99%)
Gene names: JW0717, b0728, sucC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 3 x COA
1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P4322
Unit cell:
a: 98.82Å b: 98.82Å c: 404.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.172 0.228
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mitochondrial encephalomyopathy and retinoblastoma explained by compound heterozygosity of SUCLA2 point mutation and 13q14 deletion.
Matilainen et al. (2015)
22 other articles

2 mentions without citation

Succinyl-CoA Synthetase Dysfunction as a Mechanism of Mitochondrial Encephalomyopathy: More than Just an Oxidative Energy Deficit.
Lancaster et al. (2023)
1 more