› 1ceo

X-ray diffraction
1.9Å resolution

CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN

Released:
DOI: 10.2210/pdb1ceo/pdb
PDB entry 1ceo coloured by chain, front view.
PDB entry 1ceo coloured by chain, side view.
PDB entry 1ceo coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
The crystal structure of a family 5 endoglucanase mutant in complexed and uncomplexed forms reveals an induced fit activation mechanism.
Domínguez R, Souchon H, Lascombe M, Alzari PM
J Mol Biol 257 1042-51 (1996)
PMID: 8632467

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase C Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 40.95 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2S3 (Residues: 1-343; Coverage: 100%)
Gene name: celC
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 51.4Å b: 84.33Å c: 87.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.222
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural and sequence-based classification of glycoside hydrolases.
Henrissat et al. (1997)
3 more

9 mentions without citation

3(10) helices in channels and other membrane proteins.
Vieira-Pires et al. (2010)
8 more