› 1bh2

X-ray diffraction
2.1Å resolution

A326S MUTANT OF AN INHIBITORY ALPHA SUBUNIT

Released:
DOI: 10.2210/pdb1bh2/pdb
PDB entry 1bh2 coloured by chain, front view.
PDB entry 1bh2 coloured by chain, side view.
PDB entry 1bh2 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The A326S mutant of Gialpha1 as an approximation of the receptor-bound state.
Posner BA, Mixon MB, Wall MA, Sprang SR, Gilman AG
J Biol Chem 273 21752-8 (1998)
PMID: 9705312

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 36.14 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10824 (Residues: 32-346; Coverage: 89%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3221
Unit cell:
a: 79.584Å b: 79.584Å c: 105.556Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Heterotrimeric G protein activation by G-protein-coupled receptors.
Oldham et al. (2008)
9 more

4 mentions without citation

Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif?
Byrne et al. (2012)
3 more