› 1bgt

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE DNA MODIFYING ENZYME BETA-GLUCOSYLTRANSFERASE IN THE PRESENCE AND ABSENCE OF THE SUBSTRATE URIDINE DIPHOSPHOGLUCOSE

Released:
DOI: 10.2210/pdb1bgt/pdb
PDB entry 1bgt coloured by chain, front view.
PDB entry 1bgt coloured by chain, side view.
PDB entry 1bgt coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose.
Vrielink A, Rüger W, Driessen HP, Freemont PS
EMBO J 13 3413-22 (1994)
PMID: 8062817

Function and Biology Details

Reaction catalysed: 
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA beta-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.72 KDa
Source organism: Escherichia virus T4
Expression system: Not provided
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Sequence domains: Bacteriophage T4 beta-glucosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 152.88Å b: 52.25Å c: 53.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 not available
Expression system: Not provided

Quick links

Citations

40 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
39 more

3 mentions without citation

Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases.
Gastinel et al. (2001)
2 more