› 1b04

X-ray diffraction
2.8Å resolution

STRUCTURE OF THE ADENYLATION DOMAIN OF AN NAD+ DEPENDENT LIGASE

Released:
DOI: 10.2210/pdb1b04/pdb
PDB entry 1b04 coloured by chain, front view.
PDB entry 1b04 coloured by chain, side view.
PDB entry 1b04 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of the adenylation domain of an NAD+-dependent DNA ligase.
Singleton MR, Håkansson K, Timson DJ, Wigley DB
Structure 7 35-42 (1999)
PMID: 10368271

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 35.83 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O87703 (Residues: 1-318; Coverage: 48%)
Gene names: lig, ligA
Sequence domains: NAD-dependent DNA ligase adenylation domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P43212
Unit cell:
a: 95.72Å b: 95.72Å c: 225.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.31
Expression system: Escherichia coli BL21

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Citations

6 review citations

Bacterial DNA ligases.
Wilkinson et al. (2001)
5 more

4 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
3 more