› 1auo

X-ray diffraction
1.8Å resolution

CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS

Released:
DOI: 10.2210/pdb1auo/pdb
PDB entry 1auo coloured by chain, front view.
PDB entry 1auo coloured by chain, side view.
PDB entry 1auo coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Crystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity.
Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW
Structure 5 1571-84 (1997)
PMID: 9438866

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylesterase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 23.9 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53547 (Residues: 1-218; Coverage: 100%)
Gene name: estB
Sequence domains: Phospholipase/Carboxylesterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P41212
Unit cell:
a: 82.04Å b: 82.04Å c: 145.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.273
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.
Jaeger et al. (1999)
6 more

21 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
20 more