› 1aha

X-ray diffraction
2.2Å resolution

THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN

Released:
DOI: 10.2210/pdb1aha/pdb
PDB entry 1aha coloured by chain, front view.
PDB entry 1aha coloured by chain, side view.
PDB entry 1aha coloured by chain, top view.
Source organism: Momordica charantia
Primary publication:
The N-glycosidase mechanism of ribosome-inactivating proteins implied by crystal structures of alpha-momorcharin.
Ren J, Wang Y, Dong Y, Stuart DI
Structure 2 7-16 (1994)
PMID: 8075985

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein momordin I Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.4 KDa
Source organism: Momordica charantia
Expression system: Not provided
UniProt:
  • Canonical: P16094 (Residues: 24-269; Coverage: 94%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R3
Unit cell:
a: 132.2Å b: 132.2Å c: 38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 not available
Expression system: Not provided

Quick links

Citations

4 review citations

The history of ricin, abrin and related toxins.
Olsnes S. (2004)
3 more

14 mentions without citation

Plant Ribosome-Inactivating Proteins: Progesses, Challenges and Biotechnological Applications (and a Few Digressions).
Fabbrini et al. (2017)
13 more