› 1af5

X-ray diffraction
3Å resolution

GROUP I MOBILE INTRON ENDONUCLEASE

Released:
DOI: 10.2210/pdb1af5/pdb
PDB entry 1af5 coloured by chain, front view.
PDB entry 1af5 coloured by chain, side view.
PDB entry 1af5 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The structure of I-Crel, a group I intron-encoded homing endonuclease.
Heath PJ, Stephens KM, Monnat RJ, Stoddard BL
Nat Struct Biol 4 468-76 (1997)
PMID: 9187655

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA endonuclease I-CreI Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 4-138; Coverage: 83%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P321
Unit cell:
a: 78.2Å b: 78.2Å c: 67.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.248 0.248 0.373
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Homing endonucleases: keeping the house in order.
Belfort et al. (1997)
8 more

2 mentions without citation

Identification of protein oligomerization states by analysis of interface conservation.
Elcock et al. (2001)
1 more