› 1ae2

X-ray diffraction
2Å resolution

MUTANT L32R OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Released:
DOI: 10.2210/pdb1ae2/pdb
PDB entry 1ae2 coloured by chain, front view.
PDB entry 1ae2 coloured by chain, side view.
PDB entry 1ae2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography.
Su S, Gao YG, Zhang H, Terwilliger TC, Wang AH
Protein Sci 6 771-80 (1997)
PMID: 9098886

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-Binding protein G5P Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69543 (Residues: 1-87; Coverage: 100%)
Gene name: V
Sequence domains: Helix-destabilising protein
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 76.01Å b: 28.03Å c: 42.4Å
α: 90° β: 103.1° γ: 90°
R-values:
R R work R free
0.223 0.223 0.326
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and assembly of filamentous bacteriophages.
Marvin et al. (2014)
1 more

4 mentions without citation

Intrinsic evaluation of text mining tools may not predict performance on realistic tasks.
Caporaso et al. (2008)
3 more