› 1a99

X-ray diffraction
2.2Å resolution

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

Released:
DOI: 10.2210/pdb1a99/pdb
PDB entry 1a99 coloured by chain, front view.
PDB entry 1a99 coloured by chain, side view.
PDB entry 1a99 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity.
Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K
J Biol Chem 273 17604-9 (1998)
PMID: 9651355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine-binding periplasmic protein PotF Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 344 amino acids
Theoretical weight: 38.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31133 (Residues: 27-370; Coverage: 100%)
Gene names: JW0838, b0854, potF
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand PUT 4 x PUT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 269.4Å b: 82.33Å c: 93.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.233
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Polyamine transport in bacteria and yeast.
Igarashi et al. (1999)
4 more

12 mentions without citation

Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis.
Park et al. (2007)
11 more