› 1a80

X-ray diffraction
2.1Å resolution

Native 2,5-DIKETO-D-GLUCONIC acid reductase a from CORYNBACTERIUM SP. complexed with nadph

Released:
DOI: 10.2210/pdb1a80/pdb
PDB entry 1a80 coloured by chain, front view.
PDB entry 1a80 coloured by chain, side view.
PDB entry 1a80 coloured by chain, top view.
Source organism: Corynebacterium sp.
Primary publication:
Crystal structure of 2,5-diketo-D-gluconic acid reductase A complexed with NADPH at 2.1-A resolution.
Khurana S, Powers DB, Anderson S, Blaber M
Proc Natl Acad Sci U S A 95 6768-73 (1998)
PMID: 9618487

Function and Biology Details

Reaction catalysed: 
2-dehydro-L-gulonate + NADP(+) = 2,5-didehydro-D-gluconate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,5-diketo-D-gluconic acid reductase A Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 30.02 KDa
Source organism: Corynebacterium sp.
Expression system: Tatumella citrea
UniProt:
  • Canonical: P06632 (Residues: 2-278; Coverage: 100%)
Gene name: dkgA
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 35.7Å b: 55.8Å c: 74.7Å
α: 90° β: 92.2° γ: 90°
Expression system: Tatumella citrea

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Citations

1 review citation

Microbial aldo-keto reductases.
Ellis EM. (2002)
29 other articles

14 mentions without citation

HIV-1 gag: an emerging target for antiretroviral therapy.
Tedbury et al. (2015)
13 more