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Vitrification

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Cryogen:    
Humidity (%): e.g. 90 (percentage)
Cryogen temperature (K): e.g. 120 (in kelvin)
Apparatus:    
Protocol: e.g. Blot for 2 seconds before plunging
Time-resolved protocol: e.g. Vitrified 30 msec after spraying with effector
Details: e.g. Vitrification carried out in argon atmosphere

Cryogen

In order to preserve it's hydrated state the biological sample is quickly frozen by immersion in the cryogen for vitrification.

Example: ETHANE
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Alternative cryogen

If the cryogen used is not found in the drop-down list, report it in this text area.

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Vitrification humidity

The humidity (%) in the vicinity of the vitrification process.

Range: 0 to 100 (%)
Example: 90.0
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Cryogen temperature

The liquid cryogen temperature (Kelvin scale).

Range: 0 to 310 (kelvin) Example: 120
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Vitrification apparatus

The type of instrument used in the vitrification process.

Example: Reichert plunger
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Alternative vitrification apparatus

If the vitrification apparatus used is not found in the drop-down list, report it in this text area.

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Vitrification method

The procedure for vitrification. Example: A small vial of ethane (the cryogen) is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.

Example: Blot twice for 3 seconds
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Time-resolved protocol

The length of time after an event effecting the sample that vitrification was induced and a description of the event.

Example: Vitrified 30 msec after spraying with effector
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Vitrification Details

Additional details relating to vitrification.

Example: Vitrification carried out in argon atmosphere
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