bimolecular fluorescence complementation

Go to external page http://purl.obolibrary.org/obo/MI_0809


The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy. [ http://www.ncbi.nlm.nih.gov/pubmed/11983170 ]

This is just here as a test because I lose it

Term information

Subsets

PSI-MI_slim

definition

The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.

has exact synonym

bifc
green fluorescence protein complementation assay
gfp complementation

id

MI:0809