JSON

luminescence based mammalian interactome mapping

^ http://purl.obolibrary.org/obo/MI_0729


This strategy uses a luciferase enzyme fused to proteins of interest, which are then coexpressed with individual epitope-tagged partners in mammalian cells. Their interactions are determined by performing an enzymatic assay on anti-tag immunoprecipitates. [ PMID:15761153 ]

Term info

Subsets

PSI-MI_slim

definition

This strategy uses a luciferase enzyme fused to proteins of interest, which are then coexpressed with individual epitope-tagged partners in mammalian cells. Their interactions are determined by performing an enzymatic assay on anti-tag immunoprecipitates.

id

MI:0729

Term relations