electrophoretic mobility supershift assay

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The EMSA supershift is a EMSA experiment carried out using a third lane loaded with the radiolabeled nucleic acid, a protein mixture and an antibody for a specific protein. If an extra retardation is observed, this is due to the formation of a larger complex including the antibody. By this approach, at least one protein of the complex is directly identified. [ http://www.ncbi.nlm.nih.gov/pubmed/12169687 ]

This is just here as a test because I lose it

Term information

Subsets

PSI-MI_slim

definition

The EMSA supershift is a EMSA experiment carried out using a third lane loaded with the radiolabeled nucleic acid, a protein mixture and an antibody for a specific protein. If an extra retardation is observed, this is due to the formation of a larger complex including the antibody. By this approach, at least one protein of the complex is directly identified.

has exact synonym

emsa supershift

id

MI:0412

Term relations