This approach is used to identify the residues that are involved in an interaction. Several variants of the native protein are prepared by sequentially mutating each residue of interest to an alanine. The mutated proteins are expressed and probed in the binding assay. [ http://www.ncbi.nlm.nih.gov/pubmed/14755292 ]

This is just here as a test because I lose it

Term information

Subsets

PSI-MI_slim

definition

This approach is used to identify the residues that are involved in an interaction. Several variants of the native protein are prepared by sequentially mutating each residue of interest to an alanine. The mutated proteins are expressed and probed in the binding assay.

id

MI:0005

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